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Ifnγ Matched Elisa Antibody Pair Set, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transcription factor staining buffer  (Miltenyi Biotec)
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Cellular phenotyping of OX40L + cells in melanoma tumors. (a) OX40L expression on SOX10 + melanoma cells was manually assessed in 30 tumors (5–10 ROIs per tumor; 3 mm² per ROI). Representative images of membrane-associated OX40L expression and a summary of prevalence across tumors are shown. Rare OX40L + melanoma cells (<10 cells per ROI) were classified as “scattered” or “focally clustered,” whereas “widespread” distribution was defined as OX40L expression in ~10% of SOX10 + melanoma cells per ROI. (b) Exploratory OX40L immune cell phenotyping was performed on serial tumor sections from three melanoma cases. Representative multiplex immunofluorescence images illustrate OX40L expression in macrophages (CD68 + , CD163 + ), dendritic cells (CD11c + ), CD4 + T cells, CD8 + T cells, and <t>Foxp3</t> + regulatory T cells. (c) Proportions of immune cell subsets within the TME across three tumors (8–15 ROIs per tumor). (d) Violin plots depicting the proportion of OX40L + cells within each immune subset. Mac, macrophages; DC, dendritic cells; T, T cells. Each dot represents one ROI, dot colors represent tumor sample, and mean and median values are shown in red squares and black diamonds.
Foxp3 Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cellular phenotyping of OX40L + cells in melanoma tumors. (a) OX40L expression on SOX10 + melanoma cells was manually assessed in 30 tumors (5–10 ROIs per tumor; 3 mm² per ROI). Representative images of membrane-associated OX40L expression and a summary of prevalence across tumors are shown. Rare OX40L + melanoma cells (<10 cells per ROI) were classified as “scattered” or “focally clustered,” whereas “widespread” distribution was defined as OX40L expression in ~10% of SOX10 + melanoma cells per ROI. (b) Exploratory OX40L immune cell phenotyping was performed on serial tumor sections from three melanoma cases. Representative multiplex immunofluorescence images illustrate OX40L expression in macrophages (CD68 + , CD163 + ), dendritic cells (CD11c + ), CD4 + T cells, CD8 + T cells, and <t>Foxp3</t> + regulatory T cells. (c) Proportions of immune cell subsets within the TME across three tumors (8–15 ROIs per tumor). (d) Violin plots depicting the proportion of OX40L + cells within each immune subset. Mac, macrophages; DC, dendritic cells; T, T cells. Each dot represents one ROI, dot colors represent tumor sample, and mean and median values are shown in red squares and black diamonds.
Elisa Antibody Pair Set Kit, supplied by Sino Biological, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cellular phenotyping of OX40L + cells in melanoma tumors. (a) OX40L expression on SOX10 + melanoma cells was manually assessed in 30 tumors (5–10 ROIs per tumor; 3 mm² per ROI). Representative images of membrane-associated OX40L expression and a summary of prevalence across tumors are shown. Rare OX40L + melanoma cells (<10 cells per ROI) were classified as “scattered” or “focally clustered,” whereas “widespread” distribution was defined as OX40L expression in ~10% of SOX10 + melanoma cells per ROI. (b) Exploratory OX40L immune cell phenotyping was performed on serial tumor sections from three melanoma cases. Representative multiplex immunofluorescence images illustrate OX40L expression in macrophages (CD68 + , CD163 + ), dendritic cells (CD11c + ), CD4 + T cells, CD8 + T cells, and <t>Foxp3</t> + regulatory T cells. (c) Proportions of immune cell subsets within the TME across three tumors (8–15 ROIs per tumor). (d) Violin plots depicting the proportion of OX40L + cells within each immune subset. Mac, macrophages; DC, dendritic cells; T, T cells. Each dot represents one ROI, dot colors represent tumor sample, and mean and median values are shown in red squares and black diamonds.
Iga Elisa Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vibrance anti fade hard set mounting medium  (Vector Laboratories)
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Cellular phenotyping of OX40L + cells in melanoma tumors. (a) OX40L expression on SOX10 + melanoma cells was manually assessed in 30 tumors (5–10 ROIs per tumor; 3 mm² per ROI). Representative images of membrane-associated OX40L expression and a summary of prevalence across tumors are shown. Rare OX40L + melanoma cells (<10 cells per ROI) were classified as “scattered” or “focally clustered,” whereas “widespread” distribution was defined as OX40L expression in ~10% of SOX10 + melanoma cells per ROI. (b) Exploratory OX40L immune cell phenotyping was performed on serial tumor sections from three melanoma cases. Representative multiplex immunofluorescence images illustrate OX40L expression in macrophages (CD68 + , CD163 + ), dendritic cells (CD11c + ), CD4 + T cells, CD8 + T cells, and <t>Foxp3</t> + regulatory T cells. (c) Proportions of immune cell subsets within the TME across three tumors (8–15 ROIs per tumor). (d) Violin plots depicting the proportion of OX40L + cells within each immune subset. Mac, macrophages; DC, dendritic cells; T, T cells. Each dot represents one ROI, dot colors represent tumor sample, and mean and median values are shown in red squares and black diamonds.
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bioplex 2200 torc igg kit  (Bio-Rad)
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Cellular phenotyping of OX40L + cells in melanoma tumors. (a) OX40L expression on SOX10 + melanoma cells was manually assessed in 30 tumors (5–10 ROIs per tumor; 3 mm² per ROI). Representative images of membrane-associated OX40L expression and a summary of prevalence across tumors are shown. Rare OX40L + melanoma cells (<10 cells per ROI) were classified as “scattered” or “focally clustered,” whereas “widespread” distribution was defined as OX40L expression in ~10% of SOX10 + melanoma cells per ROI. (b) Exploratory OX40L immune cell phenotyping was performed on serial tumor sections from three melanoma cases. Representative multiplex immunofluorescence images illustrate OX40L expression in macrophages (CD68 + , CD163 + ), dendritic cells (CD11c + ), CD4 + T cells, CD8 + T cells, and <t>Foxp3</t> + regulatory T cells. (c) Proportions of immune cell subsets within the TME across three tumors (8–15 ROIs per tumor). (d) Violin plots depicting the proportion of OX40L + cells within each immune subset. Mac, macrophages; DC, dendritic cells; T, T cells. Each dot represents one ROI, dot colors represent tumor sample, and mean and median values are shown in red squares and black diamonds.
Bioplex 2200 Torc Igg Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bioplex 2200mmrv igg kit  (Bio-Rad)
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Cellular phenotyping of OX40L + cells in melanoma tumors. (a) OX40L expression on SOX10 + melanoma cells was manually assessed in 30 tumors (5–10 ROIs per tumor; 3 mm² per ROI). Representative images of membrane-associated OX40L expression and a summary of prevalence across tumors are shown. Rare OX40L + melanoma cells (<10 cells per ROI) were classified as “scattered” or “focally clustered,” whereas “widespread” distribution was defined as OX40L expression in ~10% of SOX10 + melanoma cells per ROI. (b) Exploratory OX40L immune cell phenotyping was performed on serial tumor sections from three melanoma cases. Representative multiplex immunofluorescence images illustrate OX40L expression in macrophages (CD68 + , CD163 + ), dendritic cells (CD11c + ), CD4 + T cells, CD8 + T cells, and <t>Foxp3</t> + regulatory T cells. (c) Proportions of immune cell subsets within the TME across three tumors (8–15 ROIs per tumor). (d) Violin plots depicting the proportion of OX40L + cells within each immune subset. Mac, macrophages; DC, dendritic cells; T, T cells. Each dot represents one ROI, dot colors represent tumor sample, and mean and median values are shown in red squares and black diamonds.
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Cellular phenotyping of OX40L + cells in melanoma tumors. (a) OX40L expression on SOX10 + melanoma cells was manually assessed in 30 tumors (5–10 ROIs per tumor; 3 mm² per ROI). Representative images of membrane-associated OX40L expression and a summary of prevalence across tumors are shown. Rare OX40L + melanoma cells (<10 cells per ROI) were classified as “scattered” or “focally clustered,” whereas “widespread” distribution was defined as OX40L expression in ~10% of SOX10 + melanoma cells per ROI. (b) Exploratory OX40L immune cell phenotyping was performed on serial tumor sections from three melanoma cases. Representative multiplex immunofluorescence images illustrate OX40L expression in macrophages (CD68 + , CD163 + ), dendritic cells (CD11c + ), CD4 + T cells, CD8 + T cells, and Foxp3 + regulatory T cells. (c) Proportions of immune cell subsets within the TME across three tumors (8–15 ROIs per tumor). (d) Violin plots depicting the proportion of OX40L + cells within each immune subset. Mac, macrophages; DC, dendritic cells; T, T cells. Each dot represents one ROI, dot colors represent tumor sample, and mean and median values are shown in red squares and black diamonds.

Journal: Frontiers in Immunology

Article Title: Prognostic immunological implications of OX40L expression in the tumor microenvironment of melanoma

doi: 10.3389/fimmu.2026.1745742

Figure Lengend Snippet: Cellular phenotyping of OX40L + cells in melanoma tumors. (a) OX40L expression on SOX10 + melanoma cells was manually assessed in 30 tumors (5–10 ROIs per tumor; 3 mm² per ROI). Representative images of membrane-associated OX40L expression and a summary of prevalence across tumors are shown. Rare OX40L + melanoma cells (<10 cells per ROI) were classified as “scattered” or “focally clustered,” whereas “widespread” distribution was defined as OX40L expression in ~10% of SOX10 + melanoma cells per ROI. (b) Exploratory OX40L immune cell phenotyping was performed on serial tumor sections from three melanoma cases. Representative multiplex immunofluorescence images illustrate OX40L expression in macrophages (CD68 + , CD163 + ), dendritic cells (CD11c + ), CD4 + T cells, CD8 + T cells, and Foxp3 + regulatory T cells. (c) Proportions of immune cell subsets within the TME across three tumors (8–15 ROIs per tumor). (d) Violin plots depicting the proportion of OX40L + cells within each immune subset. Mac, macrophages; DC, dendritic cells; T, T cells. Each dot represents one ROI, dot colors represent tumor sample, and mean and median values are shown in red squares and black diamonds.

Article Snippet: Cells were stained with fluorophore-conjugated antibodies against CD4, CD25, OX40, OX40L, and intracellular Foxp3 using the Foxp3 Buffer Set (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Expressing, Membrane, Multiplex Assay, Immunofluorescence

Expression of OX40L and OX40 on regulatory T cells in melanoma tumors and peripheral blood of a healthy donor. OX40L and OX40 expression were assessed on Foxp3 + regulatory T cells (Tregs) across 30 melanoma tumors using quantitative multiplex immunofluorescence analysis (10–20 ROIs per tumor; 3 mm² per ROI). (a) Representative images showing Foxp3 + Tregs within a melanoma tumor (Foxp3 + nuclei shown in orange when alone or magenta when overlaid with DAPI) co-expressing OX40L (green), OX40 (red), or both. Right panels show higher-magnification views of circled cells with individual color channels; all panels share the same scale bar, indicated in the DAPI image. (b) Box plots depicting the proportions of OX40L + , OX40 + , and double-positive Tregs across 30 tumors. The mean is indicated by a “X” mark and the median by a horizontal line (***p < 0.001, Student’s t-test). Jittered dots represent the mean value per tumor across ROIs. (c) Box plots showing the prevalence of OX40L + (green), OX40 + (red), and OX40L + /OX40L + double-positive (blue) Tregs per tumor, ordered by OX40L + frequency. Jittered dots represent individual ROIs. (d) Tregs isolated from peripheral blood of a healthy donor were enriched and expanded in vitro under IL-2/CD3/CD28 stimulation. The proportions of OX40L + and OX40 + cells within live, singlet Foxp3 + regulatory T cells, as defined in the Methods, are shown over 13 days of culture.

Journal: Frontiers in Immunology

Article Title: Prognostic immunological implications of OX40L expression in the tumor microenvironment of melanoma

doi: 10.3389/fimmu.2026.1745742

Figure Lengend Snippet: Expression of OX40L and OX40 on regulatory T cells in melanoma tumors and peripheral blood of a healthy donor. OX40L and OX40 expression were assessed on Foxp3 + regulatory T cells (Tregs) across 30 melanoma tumors using quantitative multiplex immunofluorescence analysis (10–20 ROIs per tumor; 3 mm² per ROI). (a) Representative images showing Foxp3 + Tregs within a melanoma tumor (Foxp3 + nuclei shown in orange when alone or magenta when overlaid with DAPI) co-expressing OX40L (green), OX40 (red), or both. Right panels show higher-magnification views of circled cells with individual color channels; all panels share the same scale bar, indicated in the DAPI image. (b) Box plots depicting the proportions of OX40L + , OX40 + , and double-positive Tregs across 30 tumors. The mean is indicated by a “X” mark and the median by a horizontal line (***p < 0.001, Student’s t-test). Jittered dots represent the mean value per tumor across ROIs. (c) Box plots showing the prevalence of OX40L + (green), OX40 + (red), and OX40L + /OX40L + double-positive (blue) Tregs per tumor, ordered by OX40L + frequency. Jittered dots represent individual ROIs. (d) Tregs isolated from peripheral blood of a healthy donor were enriched and expanded in vitro under IL-2/CD3/CD28 stimulation. The proportions of OX40L + and OX40 + cells within live, singlet Foxp3 + regulatory T cells, as defined in the Methods, are shown over 13 days of culture.

Article Snippet: Cells were stained with fluorophore-conjugated antibodies against CD4, CD25, OX40, OX40L, and intracellular Foxp3 using the Foxp3 Buffer Set (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Expressing, Multiplex Assay, Immunofluorescence, Isolation, In Vitro